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microarray for messenger rna (mrna)  (Pathwork Diagnostics Inc)

 
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    Pathwork Diagnostics Inc microarray for messenger rna (mrna)
    Microarray For Messenger Rna (Mrna), supplied by Pathwork Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray for messenger rna (mrna)/product/Pathwork Diagnostics Inc
    Average 90 stars, based on 1 article reviews
    microarray for messenger rna (mrna) - by Bioz Stars, 2026-05
    90/100 stars

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    Profiling of NAC1-regulated genes in prostate cancer cells. (A) Hierarchical clustering of mRNA by the Z -score method. The results are displayed as a heat map, in which red indicates relatively high expression and green denotes relatively low expression. (B) Pathways corresponding to the up-regulated transcripts. (C) Pathways corresponding to down-regulated transcripts. (D) Western blots and RT-qPCR shown a significant reduction of NAC1 protein and mRNA in DU-145 cells transfected with NAC1 siRNAs compared with those transfected with NC (***P<0.001) (E). RT-qPCR was performed to validate the NAC1-regulated genes identified by microarray analysis in DU-145 cells transfected with siNAC1-1 or siNAC1-2 and NC. All candidate genes were validated (*P<0.05,**P<0.01,***P<0.001) by both siRNAs.

    Journal: Oncology Letters

    Article Title: NAC1 promotes the migration of prostate cancer cells and participates in osteoclastogenesis by negatively regulating IFNβ

    doi: 10.3892/ol.2017.7670

    Figure Lengend Snippet: Profiling of NAC1-regulated genes in prostate cancer cells. (A) Hierarchical clustering of mRNA by the Z -score method. The results are displayed as a heat map, in which red indicates relatively high expression and green denotes relatively low expression. (B) Pathways corresponding to the up-regulated transcripts. (C) Pathways corresponding to down-regulated transcripts. (D) Western blots and RT-qPCR shown a significant reduction of NAC1 protein and mRNA in DU-145 cells transfected with NAC1 siRNAs compared with those transfected with NC (***P<0.001) (E). RT-qPCR was performed to validate the NAC1-regulated genes identified by microarray analysis in DU-145 cells transfected with siNAC1-1 or siNAC1-2 and NC. All candidate genes were validated (*P<0.05,**P<0.01,***P<0.001) by both siRNAs.

    Article Snippet: An Arraystar Human messenger RNA (mRNA) Microarray v3.0 was used and run by the service provider.

    Techniques: Expressing, Western Blot, Quantitative RT-PCR, Transfection, Microarray

    NAC1 promotes prostate cancer bone metastasis in a manner dependent on the induction of IFNβ. (A) NAC1 knockdown efficiency was verified in PC-3 cells by RT-qPCR and western blotting. (B) Genes associated with activation of osteoclasts were identified in RAW 264.7 cells by RT-qPCR and western blotting (**P<0.01, ***P<0.001). (C) The mRNA and protein levels of IFNβ were verified in PC-3 cells by RT-qPCR and western blotting (**P<0.01). (D) ELISA was performed to validate the protein level of IFNβ in the co-culture medium The differences between the siNAC1-1 or siNAC1-2 transfection groups and the NC group in the Figure were statistically significant (**P<0.01, ***P<0.001).

    Journal: Oncology Letters

    Article Title: NAC1 promotes the migration of prostate cancer cells and participates in osteoclastogenesis by negatively regulating IFNβ

    doi: 10.3892/ol.2017.7670

    Figure Lengend Snippet: NAC1 promotes prostate cancer bone metastasis in a manner dependent on the induction of IFNβ. (A) NAC1 knockdown efficiency was verified in PC-3 cells by RT-qPCR and western blotting. (B) Genes associated with activation of osteoclasts were identified in RAW 264.7 cells by RT-qPCR and western blotting (**P<0.01, ***P<0.001). (C) The mRNA and protein levels of IFNβ were verified in PC-3 cells by RT-qPCR and western blotting (**P<0.01). (D) ELISA was performed to validate the protein level of IFNβ in the co-culture medium The differences between the siNAC1-1 or siNAC1-2 transfection groups and the NC group in the Figure were statistically significant (**P<0.01, ***P<0.001).

    Article Snippet: An Arraystar Human messenger RNA (mRNA) Microarray v3.0 was used and run by the service provider.

    Techniques: Knockdown, Quantitative RT-PCR, Western Blot, Activation Assay, Enzyme-linked Immunosorbent Assay, Co-Culture Assay, Transfection